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LANCE assays are TR-FRET assays using a Europium chelate as the donor fluorophore. Either white or black plates indicated for fluorescence applications can be used, though we typically recommend white plates for LANCE assays. LANCE assays utilize time-resolved fluorescence resonance energy transfer technology (TR-FRET) to generate a fluorescent signal at 665 nm. In contrast to prompt fluorescence assays, TR-FRET assays delay the signal acquisition for 50 microseconds in order to let any autofluorescent signal from the microplate or sample decay to background.