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Early Serodiagnosis of Porcine Reproductive and Respiratory Syndrome Virus Infection of Pigs by Detection of Slow-Reacting and Complement-Requiring Neutralizing.
Sato K, Tanaka Y, Tokuhisa S, Inaba Y, Fushiki T, Matumoto M (1987) Appearance of slow-reacting and complement-requiring neutralizing.
Slow-reacting complement-requiring neutralizing (NT) antibody was detected in sera from cattle 2 weeks after infection with Akabane virus. Bovine sera obtained 3 or 4 weeks after infection contained slow-reacting noncomplement-requiring NT antibody. The slow-reacting complement-requiring NT antibody was sensitive to 2-mercaptoethanol (2-ME), whereas the slow-reacting noncomplement-requiring NT antibody was resistant to 2-ME.
XI. Differences between Slow-Reacting Complement-Requiring Neutralizing.
In this study, the plasmids containing PE-PRRS chimeric subunits were constructed that composed of porcine reproductive and respiratory syndrome virus (PRRSV) antigen moieties, a ligand moiety and a Pseudomonas exotoxin A deleted domain III (PE (?III)), and a carboxyl terminal moiety that includes a polypeptide with amino acid sequence KDEL (K3). The PE-PRRS combination vaccine can effectively induce not only PRRSV-specific INF-? cellular immunity but also a slow-reacting and complement-requiring type serum neutralizing.