What does ssDBF stand for?
ssDBF stands for single-stranded D-box-binding factor
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These single-stranded regions have all been studied in transcriptionally active promoters. Whether the single-stranded DNA binding factors also continue to bind to the transcriptionally inactive promoters and, if so, what changes take place during their transition into a transcriptionally active state, need to be studied. In addition, mechanisms involved in generating isolated single-stranded sites at specific points within a predominantly double-stranded genome need to be examined in detail.
Proteins were then transferred to nitrocellulose membranes (Schleicher & Schuell, Keene, NH, USA). These were saturated by incubation at 4 °C overnight with 10% non-fat dry milk in NaCl/Pi/0. 1% Tween 20 and then incubated with the rabbit polyclonal anti-[single-stranded D-box-binding factor (ssDBF)] serum for 60 min at room temperature. The rabbit polyclonal anti-ssDBF serum was used at a dilution of 1 : 1000 in NaCl/Pi/0.
Alignments of the translated product of the largest clone of the DBP40 gene with mouse CBF-A and chicken apoVLDL II promoter single-stranded D-box binding factor (ssDBF), the most similar sequences in the GenBank database. The shaded sequences indicate the residues which differ between DBP40 and either of the other two proteins.
In situ UV-cross-linking experiments demonstrated that CBNPα and CBNPγ interact directly with the telomeric d(CCCTAA)n repeat, whereas CBNPβ does not. Moreover, they provided information on the protein components responsible for each electrophoretic mobility-shift assay signal. Ion spray and matrix-assisted laser desorption ionization MS allowed us to identify CBNPα with single-stranded D-box-binding factor.