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What does RME stand for?

RME stands for normal rat mammary epithelial

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Currently unknown is whether breast tumours have a common cell of origin or whether different types of tumours originate from different types of cells, or whether it is a combination of these two processes [4]. Support for the second hypothesis comes from studies in which different populations of human breast epithelial cells were selected from in vitro cultures or were purified using flow cytometry and reverse-engineered into tumours of distinct subtypes using lentiviral vectors [5,6]. Similar results have been observed in a mouse model where loss of Brca1 in different epithelial cell compartments resulted in tumours with different histologies [7]. An understanding of the properties of the normal mammary epithelial.
Cell culture and treatment. Immortal normal mammary epithelial cells, MCF12A, and breast cancer cell lines MDA-MB231 and BT549 were purchased from ATCC. Primary breast tumor cells (freshly isolated from grades II–III ductal carcinoma) were purchased from Promab Inc. MCF12A cells were grown in DMEM-F12 medium supplemented with 5% horse serum, EGF (20 ng/ml), insulin (10 µg/ml), cholera toxin (1 ng/ml), hydrocortisone, and gentamycin (Sigma-Aldrich).