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What does N/A stand for?

N/A stands for non-adherent cells


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In this video, we have demonstrated the steps to isolate bone marrow and differentiate myeloid dendritic cells in vitro. Following the culture period, these cells can be visualized microscopically both as adherent cells, which often possess dendrites, and non-adherent round cells. The DCs can be further manipulated for antigen presentation by pulsing with antigen or stimulated for cytokine production and costimulatory molecule upregulation using cytokines and/or toll-like receptor ligands (for review, see Gilboa, 2008(2)).
Receptor-mediated signalling is particularly sensitive to mechanical stress; indeed, shear stresses as low as 1 dyn cm−2 have been shown to activate intracellular Erk signalling29. This effect was carefully evaluated in DISC, and cell signalling was not induced even under long durations (up to 5 min) of continuous droplet movement (Supplementary Fig. 8). Furthermore, while stressors other than shear (for example, electrical or thermal effects) were not explicitly evaluated here, previous studies50 have reported that DMF actuation has negligible effects on cell phenotype when probed for targeted (quantitative PCR for stress-related genes) and untargeted (complementary DNA microarrays) expression changes. In the experiments reported here, three adherent cell.