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This buffer contains: 10mM sodium phosphate (pH 7.4) with 2.7mM KCl, 140mM NaCl, and 1% Triton X-100. Lyse for 30 minutes at 4°C. Add lysozyme, DNase, RNase, and...
activity was not affected by catalase, lysozyme, DNase, RNase A, a-amylase, and lipase (Schillinger & LUcke, 1989; Keppler, 1992). All enzymes were applied in...
After lysozyme/Dnase/Rnase treatment the cells were lysed using a French press, and membranes were removed by ultracentrifugation (250,000 3 g, 30 min). Cell extract
0.36 mg dry weight of the washed cells was obtained from 10 ml of culture. The lysozyme-DNase-RNase (LDR) method was performed as previously reported.