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The borate buffer (BOR) of Hertz et al. (Scand J Clin Invest 1974;33:215-30), the caffeine buffer (CAF) of Vink et al. (Clin Chem 1988;34:67-70), and the combined borate-caffeine buffer (B-C) of Franzini and Cattozzo (Clin Chem 1987;33:597-9) were compared. All methods required only 10 microL of serum, were precise (between-batch CVs less than 4. 2%, analyte range 64-310 mumol/L), linear to 1000 mumol/L, and insensitive to interference from hemoglobin to 5 g/L.