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Overview | Description | Applications | Operations | Results | Publications | Imagery ISS Science for Everyone Science Objectives for Everyone Transgenic Arabidopsis Gene Expression System (TAGES) investigation is one in a pair of investigations that use the Advanced Biological Research System (ABRS) facility. TAGES uses Arabidopsis thaliana, thale cress, with sensor promoter-reporter gene constructs that render the plants as biomonitors, or an organism used to determine the quality of the surrounding environment, using real-time nondestructive Green Fluorescent Protein imagery and traditional postflight analyses.
Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a ( R esponsive to D ehydration 29A ) promoter, CBF3 ( C -repeat B inding F actor 3 ) transcription factor and cpl1-2 ( C TD p hosphatase- l ike 1 ) mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 ( P roduction of A nthocyanin P igment 1 ) transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgenic plants with only one or two of three components did not reproducibly accumulate anthocyanin pigments above the control level, transgenic cpl1 plants containing homozygous RD29a-PAP1 and RD29a-CBF3 transgenes produced 30-fold higher level of total anthocyanins than control plants upon cold treatment. Growth retardation and phytochemical production of transgenic plants were minimum under normal conditions. The flavonoid profile in cold-induced transgenic plants was determined by LC/MS/MS, which resembled that of previously reported pap1-D plants but enriched for kaempferol derivatives. These results establish the functionality of the inducible three-component gene expression system in plant metabolic engineering. Furthermore, we show that PAP1 and environmental signals synergistically regulate the flavonoid pathway to produce a unique flavonoid blend that has not been produced by PAP1 overexpression or cold treatment alone.
Ferl, Ph. D. , University of Florida, Gainesville, FL, United States Co-Investigator(s)/Collaborator(s) Anna-Lisa Paul, Ph. D. , University of Florida, Gainesville, FL, United States Developer(s) NASA Kennedy Space Center, Cape Canaveral, FL, United States Sponsoring Space Agency National Aeronautics and Space Administration (NASA) Sponsoring Organization Human Exploration and Operations Mission Directorate (HEOMD) Research Benefits Scientific Discovery, Space Exploration ISS Expedition Duration 1 September 2014 - March 2015 Expeditions Assigned 41/42 Previous ISS Missions TAGES-ISA builds upon the previously flown Plant Growth Investigations in Microgravity (PGIM) experiment which flew on STS-93, the Biological Research in Canisters (BRIC)-16 experiment which flew on STS-131, and the Transgenic Arabidopsis Gene Expression System.
The Transgenic Arabidopsis Gene Expression System (TAGES) investigation is one in a pair of investigations that use the Advanced Biological Research System (ABRS) facility. TAGES uses Arabidopsis thaliana, thale cress, with sensor promoter-reporter gene constructs that render the plants as biomonitors (an organism used to determine the quality of the surrounding environment) of their environment using real-time nondestructive Green Fluorescent Protein (GFP) imagery and traditional postflight analyses.