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To prevent the influence of overextension or underextension of SPA by fluid pressure, the tissue was not injected with any fluid under pressure during fixation. 10 Thirty step sections at 50-µm intervals were prepared in each case. Elastica-Goldner staining was performed,11 and the following were assessed: (1) severity of intimal lesions; (2) radius and medial thickness of SPA and small pulmonary veins (SPV), which are pulmonary veins with a muscular coat observed in biopsy specimens; their radius range is ˜25 µm to 250 µm; (3) radius of bronchioles, and the radius of SPA accompanying the bronchioles (supernumeral arteries12,13 that did not run parallel to the bronchioles were excluded); (4) radial alveolar counts.