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Briefly, epididymal fat tissue was minced and digested in a Krebs-Ringer bicarbonate (KRB) buffer (pH 7. 4) supplemented with 3% fatty acid–free BSA fraction V, 0. 5 mmol/L adenosine, and 1 mg/mL type I collagenase. After 40-min digestion and three washes, isolated adipocytes were resuspended in KRB supplemented with 5 mmol/L glucose and 3% fatty acid–free BSA. Suspended adipocytes (∼6,000 cells) were used for a lipolysis assay in 400 μL KRB buffer plus 3% fatty acid–free BSA, 1 unit/mL adenosine deaminase, and 100 nmol/L (-)-N6-(2-phenyl-isopropyl)-adenosine.
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38594-96-6 (-)-N6-(2-PHENYLISOPROPYL)-ADENOSINE N6-[L-2-PHENYL-ISOPROPYL]ADENOSINE [R]-PIA [R]-N6-[1-METHYL-2-PHENYLETHYL]-ADENOSINE R-(-)-N6-(2-PHENYL-ISOPROPYL)ADENOSINE (r)-adenosin.
Spinal R-phenyl-isopropyl adenosine inhibits spinal dorsal horn neurons responding to noxious heat stimulation in the absence and presence of sensitization. Sumida T1, Smith MA, Maehara Y, Collins JG, Kitahata LM.
v. ) and intraperitoneal (i. p. ) injection in conscious mice. The compounds tested were the non-selective adenosine receptor agonist 5'-N-ethyl-carboxamidoadenosine (NECA), the adenosine A1 receptor-selective agonists cyclopentyl-adenosine (CPA), N6-(9R-phenyl-isopropyl)-adenosine.