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L-ascorbic-6-14C acid with a specific activity of 3. 25 μCi/mg was prepared from D-glucose-1-14C in an overall yield of 10 %. Synthetic steps were : glucose to sorbitol, sorbose, diisopropylidene sorbose, potassium diisopropylidene 2-ketogulonate, 2-ketogulonic acid and ascorbic acid. In a similar manner L-ascorbic-5-14C acid was prepared from D-glucose-2-14C. Conversion of 2-keto-L-gulonic acid to ascorbic acid was done in 0.
Hornig D, Weber F, Wiss O. Autoradiographic distribution of (1- 14 C) ascorbic acid and (1- 14 C) dehydroascorbic acid in male guinea pigs after intravenous injection. Int J Vitam Nutr Res. 1972;42(2):223–241.