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B. How are embryonic stem cells grown in the laboratory? Growing cells in the laboratory is known as cell culture. Human embryonic stem cells (hESCs) are generated by transferring cells from a preimplantation -stage embryo into a plastic laboratory culture dish that contains a nutrient broth known as culture medium.
The uncomfortable truth is, James Thomson, who led the effort that first isolated and grew embryonic stem cells in the laboratory says the trophoblast can reform under certain circumstances. That means even after months of continuous proliferation of the cells, implantable cloned human beings of the original embryo might be forming as the stem cells are grown in petri dishes. Therefore, use of the term pluripotent is scientifically inaccurate and deliberately misleading.