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However, the longer the dendrites grow, the greater the number of PLA dots that are found in a single neuron. Because dynein is a motor that walks along the MT fiber, the NAGK-dynein complex would be expected to localize to MT fibers. Likewise, PLA signals for NAGK-DYNLRB1 (Figure 3a, arrowheads in upper panel) and NAGK-DHC ( Figure 3a, arrowheads in the lower panel) were localized to MT fibers and were distributed in somatodendritic domains. The negative control, where primary antibody was not added, showed no PLA signals (data not shown). Because we found an unexpectedly small number of PLA signals, we performed PLA in HEK293T cells with dynein light-chain.