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NM_001037339. 2 ? NP_001032416. 1 cAMP-specific 3',5'-cyclic phosphodiesterase.
3 Souness JE, Griffin M, Maslen C, et al. Evidence that cyclic AMP phosphodiesterase inhibitors suppress TNF alpha generation from human monocytes by interacting with a 'low‑affinity' phosphodiesterase 4 conformer. Br J Pharmacol. 1996;118:649‑658.
96-well microplate format permits rapid assays of large numbers of samples. The basis for the assay is the cleavage of cAMP or cGMP by a cyclic nucleotide phosphodiesterase. The 5’-nucleotide released is further cleaved into the nucleoside and phosphate by the enzyme 5’- nucleotidase. The phosphate released due to enzymatic cleavage is quantified using BIOMOL GREEN™ reagent in a modified Malachite Green assay. The kit includes Type I cyclic AMP phosphodiesterase.