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This comprehensive inventory includes B-Lymphoblastoid Cell Lines (B-LCL) from previous International Workshops, HLA heterozygous and homozygous donors, selected families, and individuals of diverse population groups. The RCB maintains stocks of purified DNA derived from these cell lines, as well as DNA reference panels that provide an extensive array of HLA and HLA-related sequence polymorphisms. The RCB also provides cloned HLA genes and B-LCL transfected with selected HLA genes, which are available on a limited basis.
This is based on processes introduced into, and subsequently developed by ECACC in the late 1980’s, for the immortalization of human lymphocytes. This process essentially comprises the separation of lymphocytes from samples of human peripheral blood and their transformation into lymphoblastoid cell lines by treatment with the tumourigenic Epstein Barr Virus (EBV). EBV transformation of human lymphocytes to form a cell line that can be continuously propagated provides an indispensable means of expanding the limited amount of cellular material that can be separated from a small volume blood specimen.
75. Howell DN, Machamer CE. Elimination of mycoplasma from human B-lymphoblastoid cell lines. Hum Immunol. 1982;5(3):233–238.
For example, T cell lines directed to Adv, CMV and EBV require an 8–12-week production process that also requires repeated rounds of in vitro stimulation with adenovector-modified monocytes and EBV-transformed B lymphoblastoid cell lines (EBV-LCLs). 3, 4 In addition, the generated lines have unpredictable specificities and are often dominated by CMV-reactive T cells, at the expense of EBV- and Adv-reactive T cells.
- Introduction There are many different cell lines that have been used in order to examine genotoxicity exposure of agents such as human gastric adenocarcinoma cell line - TMK1, human colon cancer - COL2, particularly human B lymphoblastoid cell line - TK6 is more often used. TK6 is a cell line heterozygous at the thymidine kinase locus from a patient with chronic myelogenous leukemia (CML) in T cell lineage blast crisis which included Philadelphia chromosome t(9;22)(q34;q11), an abnormality in chromosome 6 (a deletion of the long arm), and chromosome 7, for example, del(6)(q21); ins(1;-)(q21;-); del(1)(q21;q32); dic(7)(:p13? cen? q32:11.