What does DAB stand for?
DAB stands for 3,3'-diaminobenzidine tetrahydrochloride
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Antigen Background 3,3' diaminobenzidine tetrahydrochloride (DAB) is a substrate for.
The specimens are then incubated for five minutes with a protein block to suppress nonspecific binding of subsequent reagents, followed by a 15-minute incubation with an appropriately characterized and diluted mouse primary antibody or negative control reagent (user provided). This is followed by sequential 15-minute incubations with anti-mouse immunoglobulins-HRP, fluorescyl-tyramide hydrogen peroxide (amplification reagent) and anti-fluorescein-HRP. Staining is completed by a five-minute incubation with 3,3' diaminobenzidine tetrahydrochloride.
3,3′-Diaminobenzidine tetrahydrochloride hydrate for spectrophotometric det. of Se, ≥97.
No antigen retrieval was used for b-FGF antibody. The sections were incubated at room temperature for one hour with RB-222-P rabbit polyclonal antibodies reactive with VEGF-A (a subgroup of VEGF) (1:100; NeoMarkers, Fremont, CA, USA), sc-79 mouse monoclonal antibodies reactive with FGF-2 (147) (1:50; Santa Cruz Biotechnology, Inc. , Santa Cruz, California), and rabbit polyclonal antibodies reactive with eNOS (NeoMarkers, Fremont, CA, USA). After washing in phosphate-buffered saline with Tween-20, the tissues were incubated with a biotin-conjugated secondary antibody and then with a biotin-streptavidin complex for 30 min at room temperature. Reactions were visualized with 3,3-diaminobenzidine tetrahydrochloride.