What does HABA stand for?
HABA stands for 2-(4'-hydroxyazobenzene) benzoic acid
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The properties of the binding sites of avidin and streptavidin can also be correlated to weaker interactions, since the two proteins both form much weaker associations with the biomimetic dye, 2-(4'-hydroxyazobenzene) benzoic acid (HABA). Three-dimensional structures for avidin, the avidin-biotin complex and the avidin-HABA complex were elucidated in collaboration with Oded Livnah and Joel Sussman of the Structural Biology Department.
The HABA (2-(4-hydroxyazobenzene) benzoic acid) assay can be used to determine the extent of biotinylation. HABA dye is bound to avidin or streptavidin and yields a characteristic absorbance. When biotinylated proteins or other molecules are introduced, the biotin displaces the dye, resulting in a change in absorbance at 500 nm. This change is directly proportional to the level of biotin in the sample.
Michael Makler) and 19G7 (purchased from Flow Inc. , Portland, OR) developed for pLDH-based malaria diagnostic tests  were used as capture and detection antibodies, respectively (these monoclonal antibodies are now owned by AccessBio, Somerset, NJ). Biotinylation of 19G7 (10 µg/ml) was carried out using the EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Pierce Biotechnology) following the standard protocols provided by the manufacturer. The 2-(4-hydroxyazobenzene) benzoic acid.