cis -2,3-Dihydro-2,3-dihydroxybiphenyl Dehydrogenase and cis -1,2-Dihydro-1,2-dihydroxynaphathalene Dehydrogenase Catalyze Dehydrogenation of the Same Range of Substrates Barriault, D. ; Vedadi, M. ; Powlowski, J. ; Sylvestre, M. Pseudomonas putida strain G7 cis -1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase (NahB) and Comamonas testosteroni strain B-356 cis -2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase (BphB) were found to be catalytically active towards cis -2,3-dihydro-2,3-dihydroxybiphenyl (specificity factors of 501 and 5850 s −1 mM −1 respectively), cis -1,2-dihydro-1,2-dihydroxynaphthalene (specificity factors of 204 and 193 s −1 mM −1 respectively) and 3,4-dihydro-3,4-dihydroxy-2,2′,5,5′-tetrachlorobiphenyl (specificity factors of 1.
2,3-Dihydro-2,3-dihydroxybiphenyl-2,3-dehydrogenase (B2,3D) catalyzes the second step in the biphenyl degradation pathway. The nucleotide sequence of Comamonas testosteroni B-356 bphB, which encodes B2,3D, was determined. Structural analysis showed that the dehydrogenases involved in the bacterial degradation of aromatic compounds are related to each other and that their phylogenetic relationships are very similar to the relationships observed for dioxygenases that catalyze the initial reaction in the degradation pathway.
cis-2,3-Dihydro-2,3-dihydroxybiphenyl Dehydrogenase and cis-1,2-Dihydro-1,2-dihydroxynaphathalene Dehydrogenase Catalyze Dehydrogenation of the Same Range of Substrates