The urinary metabolites in guinea pigs and mice receiving a single 100-mg/kg dose of an equimolar mixture of 1-butyryl-4-[aromatic-d5]-cinnamylpiperazine (I-d5) hydrochloride and I hydrochloride (I:I-d5∥ were purified and subjected to trimethylsilyl derivatization with bis(trimethylsilyl)acetamide, followed by GLC-mass spectrometry-computer analysis. Appropriate fragment and molecular ions of unidentified metabolites were selected based on the major mass fragment ions appearing in the mass spectra of the previously identified metabolites of I to provide the mass chromatograms.
The urinary metabolites in guinea pigs and mice receiving a single 100 mg/kg dose of an equimolar mixture of 1-butyryl-4-[aromatic-d(5)]-cinnamylpiperazine (I-d(5)) hydrochloride and I hydrochloride (I:I-d(5)) were purified and subjected to trimethylsilyl derivatization with bis(trimethylsilyl)acetamide, followed by GLC-mass spectrometry-computer analysis. Appropriate fragment and molecular ions of unidentified metabolites were selected based on the major mass fragment ions appearing in the mass spectra of the previously identified metabolites of I to provide the mass chromatograms.
The urinary metabolites in guinea pigs and mice receiving a single 100 mg/kg dose of an equimolar mixture of 1-butyryl-4-[aromatic-d(5)]-cinnamylpiperazine (I-d(5)) hydrochloride and I hydrochloride (I:I-d(5)) were purified and subjected to trimethylsilyl derivatization with bis(trimethylsilyl)acetamide, followed by GLC-mass spectrometry-computer analysis. Appropriate fragment and molecular ions of unidentified metabolites were selected based on the major mass fragment ions appearing in the mass spectra of the previously identified metabolites of I to provide the mass chromatograms.